In vivo antiulcer and in vitro antioxidant activity of Barleria gibsoni was .. and characterization of polyphenol oxidase from leaves of Cleome gynandra L. Food . Protective effect of standardized extract of Cleome viscosa against Animals; Anti-Ulcer Agents/administration & dosage; Anti-Ulcer. Research Journal of Pharmacognosy and Phytochemistry. Delivering full text access to the world’s highest quality literature.
|Published (Last):||8 December 2013|
|PDF File Size:||11.89 Mb|
|ePub File Size:||7.50 Mb|
|Price:||Free* [*Free Regsitration Required]|
Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer. Ethanol EBG was screened for antiulcer activity in antiklcer ligation-induced ulcer models in Wistar rats.
In vitro antioxidant activity of the extracts was tested using 2,2-diphenylpicrylhydrazyl DPPHnitric oxide NO radical scavenging activity. Total phenol and flavonoid content in the extracts were determined spectrophotometrically. The ethanol extracts showed good antioxidant capacity in DPPH radical scavenging assay and NO radical scavenging activity when compared to standard.
These findings suggest that the leaves of B. This is the first ever report of antiulcer and antioxidant activities in B. Ethanol extract prepared from the leaves of B.
Nitric Oxide, IC The half maximal inhibitory concentration, m: Gallic acid equivalence, AlCl 3: Gastric hyperacidity is a very common global problem affecting millions of people worldwide due to an imbalance between aggressive and protective factors.
The current treatment of peptic ulcer is mainly done with H 2 receptor antagonists, proton pump inhibitors, and antimuscarinics. However, most of these treatments produce adverse reaction such as hypersensitivity, arrhythmia, impotence, gynecomastia, and hematopoietic disorders. Antioxidants apparently protect the living system from oxidative insults, which is a hallmark feature of cancer, cardiovascular disease, and diabetes.
Barleria Acanthaceae is a large genus with about species of herbs and shrubs distributed chiefly in the tropical and subtropical parts of the world. Some of the important species of this genus are Barleria prionitis, Barleria greenii, Barleria albostellata, Barleria cristata, Barleria strigosa, Barleria Tomentosaetc.
Therefore, current research is focused on the discovery of natural antiulcer and antioxidant compounds from the plants for new and safer treatment options, with fewer side effects. Thus, the objective of cleomme present investigation was to evaluate in vivo antiulcer and in vitro antioxidant activity of Barleria gibsoni leaves extract EBG.
Anfiulcer fresh leaves of the B. Ethanol, methanol, gallic acid, Folin—Ciocalteu reagent, potassium ferricyanide, trichloroacetic acid, ferric chloride, ferrous chloride, sodium nitroprusside, sulfanilamide, o-phosphoric acid, naphthylethylenediamine dihydrochloride, sodium carbonate, sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate, aluminum chloride, quercetin, L-ascorbic acid, and 2,2-diphenylpicrylhydrazyl DPPHwere obtained from HiMedia Laboratory and Research Lab, India.
All the chemicals used in this study including the solvents were of analytical grade.
The experiments were carried out on Wistar strain male adult rats, aged 12—15 weeks and weighing — m. The animals were housed in colony cages and maintained under standard environmental conditions: All experiments were carried out during the light period The collected fresh matured leaves of B.
One hundred grams powdered leaves was obtained after defatted with petroleum ether and successively extracted with ethanol using Soxhlet apparatus subsequently Phytochemical screening was carried out to identify the secondary metabolites present in defatted ethanol EBG. The total phenolic content of the EBG was determined using gallic acid equivalence as described previously. Reagent blank was prepared using distilled water.
One milliliter of Folin—Ciocalteu phenol reagent was added to the mixture and shaken. The volume was then made up to the mark.
After incubation for 90 min at room temperature, the absorbance against the reagent blank was determined at nm on spectrophotometer with a preprepared gallic acid calibration curve from 0.
The content of total flavonoid in ethanol EBG leaves was assessed using aluminum chloride colorimetric method. It was incubated at room temperature for 30 min, the absorbance of the reaction mixture was measured at nm using ultraviolet spectrometer. The standard drug ascorbic acid was used. The percentage of scavenging activity was derived using the following formula. NO antlulcer scavenging of EBG was carried out as previously described. Two milliliters of antiulfer mM sodium nitroprusside in 0.
From the incubated mixture, 0. The NO radicals scavenging activity was calculated.
Ligation was done without causing any damage to the blood supply of the stomach. Animals were allowed to recover and stabilize in individual cages and were deprived of water during postoperative period.
After 4 h of surgery, rats were sacrificed and ulcer scoring was done. The ulceration in gastric mucosa was measured and scored. A score for the ulcer was studied similar to pyloric ligation-induced ulcer model.
Scoring of ulcer was made as follows; normal stomach 0red coloration 0.
Phytochemical Screening and Anti-Ulcer Activity of Cleomo Gynandra Linn
One milliliter of the total centrifuged gastric contents from each pylorus ligated rat was analyzed for hydrogen ion concentration antiilcer titrating against 0. The experiment was done cleme triplicate. These sections were stained with hematoxylin and eosin using routine procedures. The slides were examined microscopically for pathomorphological changes such as congestion, hemorrhage, edema, and erosions using an arbitrary scale for the assessment of severity of these changes.
The gastric tissue samples were fixed in neutral buffered formalin for 24 anttiulcer. Sections of tissue from stomachs were examined histopathologically to study the antiulcerogenic activity of B.
The ethanolic EBG leaves revealed the presence of alkaloids, saponins, terpenoids, flavonoids, steroids, cardiac glycosides, tannins, aminoacids, and proteins [ Table 1 ]. The total phenolic content in B. Celome this gallic acid standard curve, the total phenolic content in B. It shows the B. High amount of flavonoids was found in EBG. The photometric evaluation of the antioxidant capacity of the ethanolic EBG leaves showed good antioxidant capacity [ Figure 3 ]. A significant decrease was observed in the DPPH radical activity due to the scavenging ability of the extracts.
A lower IC 50 value indicates a higher free radical scavenging activity. The ability of DPPH radical scavenging was higher in the EBG even significantly lower than standard ascorbic acid thus proving strong antioxidant potential of B. Sodium nitroprusside acts as a major source of NO radicals. EBG scavenge the NO formed from sodium nitroprusside by inhibiting the chromophore formation, hence absorbance decrease as the concentration increased.
In pyloric ligation-induced ulcer model, oral administration of leaves in two different doses showed significant reduction in ulcer index, gastric angiulcer, free acidity, and total acidity as compared to the control group, whereas omeprazole as reference standard drug showed a significant reduction in ulcer index, gastric volume, free acidity, and total acidity [ Table 2 ].
Antiulcer activity of ethanol extract obtained antihlcer Barleria gibsoni leaves: Ulcer is formed due to pylorus ligation which can lead to the accumulation of gastric juice in the stomach, damaging the balance of aggressive and protective factors.
On pylorus ligation, accumulated secretions and the related ulcers confirm gastric acid output to abtiulcer the basic cause of gastric ulcers. The results of the histopathological investigation of Cleomee. The ethanol EBG leaves demonstrated decreasing oxidative stress and increasing percentage preventive index compared to ascorbic acid and omeprazole, respectively.
From the present study, ethanol EBG results reveal ahtiulcer antioxidant and antuilcer activity is equipoint to that of omeprazole. The cldome of bioactive compounds from B. Nowadays, most of the people include antiulecr antioxidants as nutraceuticals or as food additives. Many medicinal plants contain higher phenolic compounds such as flavonoids, monophenols, and polyphenols. The plant derived antioxidants, especially polyphenols and flavonoids have been used to treat various anitulcer such as cancer, diabetic, aging, and prevention of cardiovascular disease.
By conversion of the unpaired electrons to paired electron by hydrazine due to the hydrogen donating ability of the extract. NO is a potent pleiotropic mediator of physiological processes such as neuronal signaling, smooth muscle relaxant, regulation of cell-mediated toxicity, and inhibition of platelet aggregation. It is a diffusible free radical which plays many roles as an effectors molecule in diverse biological systems including vasodilatation, antimicrobial, neuronal messenger, and antitumor activities.
The results of this investigation showcase the potential antiulcer and antioxidant activities of ethanol EBG. However, more work is required for the isolation and characterization of the active principles responsible for these activities. Tambolihas pursuing Ph. He has more than 16 years of teaching and research experience.
He has published and presented papers in reputed National and International journals and conferences. He is life member of APTI. National Center for Biotechnology InformationU.
Journal List Pharmacognosy Res v. Tamboli and Harinath N.
Author information Copyright and License information Disclaimer. This article has been cited by other articles in PMC.
Soxhelt extraction was carried out and extracts were subjected to qualitative phytochemical analysis. Clsome obtained by Soxhlation showed higher total phenolic and flavonoid contents.
On pylorus ligation-accumulated secretions and the related ulcers confirm gastric acid output to be the basic cause of gastric ulcers. Ethanol extract of leaves attenuated the gastric volume, free acidity, total acidity and ulcer index thus showing the anti-secretory mechanism. The results of the histopathological investigation of Barleria gibsoni leaves for antiulcer effects using pylorus ligation induced ulcer model in rats laid credence to traditional use of the plant leaves in ulcer treatment.
The ethanol extract of leaves demonstrated increase in percentage preventive index cleoe to omeprazole respectively. From the present study cleo,e reveals the antiulcer activity of ethanol extract wntiulcer which is comparable to that of Omeprazole. AcanthaceaeBarleria gibsonigallic acid, L-ascorbic acid, pylorus ligation-induced ulcer model, quercetin.
Chemicals Ethanol, methanol, gallic acid, Folin—Ciocalteu reagent, potassium ferricyanide, trichloroacetic acid, ferric chloride, ferrous chloride, sodium nitroprusside, sulfanilamide, o-phosphoric acid, naphthylethylenediamine dihydrochloride, sodium carbonate, sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate, aluminum chloride, quercetin, L-ascorbic acid, and 2,2-diphenylpicrylhydrazyl DPPHwere obtained from HiMedia Laboratory and Research Lab, India.