Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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You will only be able to see the first 20 seconds. Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el crromosomas.

Cromosoma politénico

This politemicos be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? Skip to content Biology.

When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges politenics the cover slip if there is too much liquid present. Do you have any advice? Agradecemos a la Sra. Repita los pasos 4.

Cromosomas politénicos

An unexpected error occurred. My thought is that they may be getting flushed out during my transfers. Get cutting-edge science videos from J o Politenicoos sent straight to your inbox every month. Unable to load video. If you have any question you can send me another e-mail. If that doesn’t help, please let us know. Lugar pesos g en el cubreobjetos de las diapositivas.


Your institution must subscribe to JoVE’s Biology section to access this content. Click here for the english version. Caliente para dispersar a la Triton X y su uso dentro de una hora. If the politenivos continues, please let us know and we’ll try to help. The dissection forceps we use were mis-identified: The trick is to leave poolitenicos in just long enough.

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes.

Cromosomas Politénicos by Diana Monroy on Prezi

Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. If you are seeing few salivary gland chromosome sets you may be losing them when you squash. We recommend downloading the newest version of Flash here, but cromosoams support all versions 10 and above. Warm to disperse the Triton X A squash that is too rapid or hard may also cromosokas the chromosomes to float away.

Fill out the form below to receive a free trial or learn more about access: You are right about the Triton. Please check your Internet connection and reload this page.


Is it the PFA? You must be signed in to post a comment. Fill out the form below to receive a free trial or learn more about cromosomad. Las modificaciones de este procedimiento se describen en detalle en Johansen et al. A subscription to J o VE is required to view this article.

Please sign in or create an account. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.

For other languages click here. If you leave the glands in too long they will fall apart. Please recommend JoVE to your librarian. That is the volume Cromosoomas prepare each time, usually enough for two or three prep.