El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Cultivo y fusion de protoplastos [1990]

This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. None of the plants had a similar banding pattern in relation to each one of the parents. Instpp Everything you need to know about selling on Stuvia. Your fellow students know exactly where the pitfalls lie and what the key elements will protoplasttos to your success in that module.

Tema 9. Cultivo de protoplastos e hibridación somática – Tercero Biotecnología. – Stuvia

These results showed that all hybrids were aneuploids Fig. New York, NY Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

Due to that, fused and unfused protoplasts were cultured together. The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S. With their assistance, you can become well prepared and can avoid having to retake exams. This value is different from that obtained by Mattheij et al. The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.


A revised medium for rapid growth bioassays with tobacco tissue culture. A slot-blot hybridization method for screening somatic hybrids. For the obtained somatic hybrid plants, the chromosome number varied from Table 2.

Root tips were stained by lacto-propionic orcein. Plant Cell Tissue Organ Cult Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

Literature cited Austin S. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.

Final concentration was protoplasts per millilitre. For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications.

Somaclonal variation in potato. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur. The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.

Protoplast fusion, culture and regeneration. The osmolality of this medium was fixed at mOsm. None of the regenerants had 72 chromosomes, which results from the protoplasts fusion of a tetraploid with a diploid genome.


Protoplasts were precipitated by centrifugation at rpm during 10 minutes. RAPD and chromosomal analysis of the hybrids are also presented. Chromosome analysis The numbers of chromosome of S. The wild species S. This technique has created novel cellular genome configurations by combining sexually incongruent species. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

These bands also appeared in the fusion regenerants Figs. Protoplast-fusion derived Solanum cybrids: The other parent, the wild species S. Prior to fusion, protoplasts from S. With the study guides and notes written by fellow students, you are guaranteed to be properly prepared for your exams.

Shoot regeneration started twelve weeks after calli were transferred to MS medium. The effect of initial culture media. RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L. Improvements in regeneration from protoplasts of potato and studies on chromosome stability.

Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats. Earn while you study.