LAURA BLOSSER – ACADEMIC ADVISOR HEALTH SCIENCES. - ppt download
We encourage you to use this online scheduler to make an appointment but you can also use it to see if your advisor is available. When we are at a meeting. Cover: Assistant Professor Laura Andrews leads the Medical a reality. Get involved, stop by and visit when you are in New Haven, and contact me . practice that YSN is able to meet its mission-stated goal: better health 10(1), Knobf, M. T., Juarez, G., Lee, S. K., Sun, V. .. N. Starr & C. Blosser (Eds.), Pediatric. Oct 8, All life tables included in the database meet quality inclusion criteria whereby Christopher D Blosser*, Megan A Bohensky*, Rohan Borschmann*, Dipan Peter Njenga Keiyoro*, Laura Kemmer*, Andrew Haddon Kemp*, Andre Baltimore, MD, USA (B X Tran PhD); Help Me See, Inc, New York, NY.
His later years spent in Chapel Hill gave rise to his deep commitment to the idea that individuals should do whatever is in their power to improve the lives of others. This was his mantra, and he lived it fully.
Chris had a small family that adored him, and also a larger extended family of friends that includes almost everyone who ever met him. A hallmark characteristic of Chris's was his insatiable curiosity and keen intellect. During the 10 years he was on staff for an interim year he was a writer for the Academy of Natural Sciences in Philadelphia, Pennsylvaniahe discovered a love for environmental reporting and a passion for the work of EHP. It became a personal mission for him to improve the lives of people in his own and the larger global community.Meet Me In Montana by Dan Seals & Marie Osmond (Live)
He was also a skilled amateur photographer, and recorded the world around him in intimate and compelling detail. He was set to further expand these gifts to make the world a far better place; he had been recently accepted to four different law schools, including the University of Hawaii and Tulane University in New Orleans, where he was offered the Dean's Scholarship.
His plan was to study environmental and international law so that he could dedicate his life to empowering those around the world who are powerless to avoid environmental injury.
Chris gave humbly, generously, and often of himself to both friend and stranger alike. He was, in his heart, a citizen of the world. This led him to travel as often and as far as he could, fearlessly embracing new people and experiences as he went. His philosophy was always to give people — and life — the benefit of the doubt.
He loved to do anything outdoors, and was learning to be a pilot; there seemed to be no limit he would not challenge. In the end, it is Chris's unadulterated enthusiasm for life that will be most remembered, and it is fitting that his generous donation of his organs has and will continue to bring new chances at life to many, many people.
He would be pleased to know that parts of him will go on to live in the bodies of these people and bring them health, peace, and well-being. It is just as sure that Chris's spirit will continue to live in the hearts and minds of those who knew and loved him best.
Memorial donations may be made to the Samaritan's Purse Emergency Fund for the rebuilding of New Orleans or to the Society of Environmental Journalists Endowment for the support of high-quality, unbiased news coverage of environmental issues.
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Anyone who has ever known Michael Rivlin knows what an extraordinary person he was. His journalism was both elegant and fearless. Everything Michael did was of the highest quality and integrity.
Michael was more than just a superb journalist.
LAURA BLOSSER – ACADEMIC ADVISOR HEALTH SCIENCES.
He came to journalism in mid-career, after spending some time in advertising, and grew to believe deeply in the cause of improving environmental journalism by building a network of committed environmental journalists. Anyone who has paid even the slightest attention to the SEJ-talk listserv over the last few years has benefited from his frequent postings.
Michael knew a lot about a lot of different things, and he was always generous about sharing what he knew, especially with younger journalists who he sensed could especially benefit from his help. Michael could also be prickly when he felt someone's rights were being trampled in our community, and many of us learned to appreciate that quality as well.
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Skeptically, I asked him if he knew what he was in for. He just smiled and said, "If I did, I wouldn't be suggesting it. That conference, which included more than 20 panels and field trips and attracted more than people, was a Rivlin tour de force. It reflected everything Michael believed: Now Michael is gone. For those of us who knew him, it is a real loss. For those of you who didn't know Michael, take my word for it, it's a loss for you too.
We'll keep you posted about any plans for a memorial service or for charitable contributions in Michael's memory.
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- What we talk about when we talk about nanoclusters
By Kathrin Day Lassila: May 31, I met Michael inbut when I remember him, it's not in years but in stories. That was when I encountered the trademark Rivlin lede, with its beguiling narrative setup: Later, Michael persuaded me to let him profile Ward Stone, the environmentally minded New York State wildlife pathologist. It was one of the best profiles I'd ever been privileged to publish, containing the best quote I may ever publish.
Michael told Stone that the state administrators in Albany who paid Stone's salary considered him a loose cannon, and Stone protested, "I'm not a loose cannon. I know exactly where I'm firing, and usually it's at them! His reporting zeal and his ability to dog his quarry through boxes of documents and mazes of policy made him perfect for it.
After the story came out, it was picked up by Harper's and dozens of other alternative and progressive publications.
Almost three years later, it still comes up when you Google Amicus. Michael and I spent hours on the phone together whenever he was working on a story for us. Most of it was planning strategy or negotiating wording "Kathrin, Kathrin," he would say, in a tone of patient, generous wisdom, when trying to talk me into or out of somethingbut plenty of it-especially late at night, when we were both tired and worn out by bioaccumulation or sprawl statistics-was just the two of us shooting the breeze.
When he felt at ease he was a voluble and entertaining talker, with great stories to tell. He was also irascible; in the time I knew him I also knew of a number of people who were feuding with him or had feuded with him. The problem, however, becomes mathematically ill-posed, whenever high spatial frequencies in shall be uncovered, leading to the classical diffraction-limit in microscopy. In that case, the point spread functions of the individual emitters overlap strongly, and marginal fluctuations of the recorded signals on the camera have a decisive impact on the obtained shape of The general idea behind SMLM is the consideration of additional parameters, which allows for singling out a subset of dye molecules.
A single image then contains only signals from a small fraction of all dye molecules, which are likely separated far enough to avoid any signal overlap. By tuning the additional parameter deliberately or stochastically and recording multiple images, one can ultimately collect signals from all dye molecules within the sample.
To our knowledge, the first realization of this idea was demonstrated in a study by van Oijen and colleagues, in which the narrow and distinct lines in the excitation spectrum of fluorophores at low temperature were used to single out individual dye molecules within a sample of rather high densities of dyes [ 26 ]. The distribution function of signals then becomes where describes the probability that the molecule i will be detected at the excitation frequency Ideally, is essentially zero over the whole spectral region except for a single narrow spike, where the particular molecule is at resonance with the excitation laser.
Thereby, the resulting signal density on each image becomes low enough to preclude overlapping point spread functions. The deconvolution problem hence gets massively simplified to the problem of finding the emitter positions of well-separated signals. Such problems are well known from the field of single particle tracking [ 27 ]; particularly, the center position of a noise-limited signal distribution can be determined with accuracy far below its actual width [ 28 ], thereby paving the way for circumventing the resolution limit of light microscopy.
The observation of blinking [ 29 ] or photoswitchable fluorophores [ 30 — 32 ] gave rise to new and simpler concepts, which do not depend on observations at low temperatures: In that case, we can rewrite the spatial emitter distribution function as where is a Boolean function describing time-dependent fluctuations between dark and active state.
Here, time is measured in units of the frame number within the respective image sequence. Note that for our formalism, the physical principle behind the blinking is not important.
SMLM variants include points accumulation for imaging in nanoscale topography PAINT [ 36 — 38 ], which utilizes the transient binding of a fluorescently labeled probe to its target, and binding-activated localization microscopy BALM [ 39 ], where the binding of the probe additionally increases its brightness.
Generation of localization maps in SMLM. Labeled protein molecules are sequentially switched between fluorescent indicated by color and non-fluorescent dark states.
Localization maps contain the positions of all observed single molecule signals. Standard image High-resolution image Export PowerPoint slide Ideally, each emitter is imaged only once.
In this case, would be zero except for a single spike with length of one image, where the fluorophore is active. In practice, however, emissive states of a single dye molecule with varying length and interjacent dark periods may be spread over the whole imaging sequence [ 4041 ], leading to repeated detection of the same molecule i.
In contrast, undercounting arises e. The parameter can be used to account for both overcounting and undercounting: Thompson et al provided a first comprehensive analysis of the dependencies of localization errors on parameters like signal intensity, background, and pixel width [ 42 ]. Under ideal conditions i. Later, localization algorithms were developed, which achieve localization precision at the Cramer—Rao bound [ 4546 ]. See [ 47 ] for a review on various issues related to single fluorophore localization.
To obtain an SMLM image, researchers construct localization maps, which are visualized using one of a variety of rendering algorithms [ 48 — 50 ].